- Guo, Q., Wu, D., Fan, C., Peng, S., Guan, H., Shan, Z., & Teng, W. (2018). Iodine excess did not affect the global DNA methylation status and DNA methyltransferase expression in T and B lymphocytes from NOD. H-2 h4 and Kunming mice. International Immunopharmacology, 55, 151-157.
- Xue, M., Ji, X., Liang, H., Liu, Y., Wang, B., Sun, L., & Li, W. (2018). The effect of fucoidan on intestinal flora and intestinal barrier function in rats bearing breast cancer. Food & Function.
- Su, X., Tang, W., Luan, Z., Yang, Y., Wang, Z., Zhang, Y., ... & Yuan, H. Protective effect of miconazole on rat myelin sheaths following premature infant cerebral white matter injury. Experimental and Therapeutic Medicine.
- Zhao, J., Wu, X., Wu, D., Yu, Y., Yu, Y., Wang, Y., ... & Yao, K. (2018). Embryonic Surface Ectoderm-specific Mitofusin 2 Conditional Knockout Induces Congenital Cataracts in Mice. Scientific Reports, 8(1), 1522.
- Guan, X., Lu, Y., Wang, G., Gibson, P., Chen, F., Fang, K., & Yuan, Y. (2018). The Role of Regulatory T Cell in Nontypeable Haemophilus influenzae-Induced Acute Exacerbation of Chronic Obstructive Pulmonary Disease. Mediators of Inflammation, 2018.
- Yin, X., Feng, L., Ma, D., Yin, P., Wang, X., Hou, S., & Feng, J. (2018). Roles of astrocytic connexin-43, hemichannels, and gap junctions in oxygen-glucose deprivation/reperfusion injury induced neuroinflammation and the possible regulatory mechanisms of salvianolic acid B and carbenoxolone. Journal of Neuroinflammation, 15(1), 97.
- Lu, J., Fang, K., Wang, S., Xiong, L., Zhang, C., Liu, Z. & Wang, F. (2018). Anti-Inflammatory Effect of Columbianetin on Lipopolysaccharide-Stimulated Human Peripheral Blood Mononuclear Cells. Mediators of Inflammation, 2018.
- Li, X., Yang, L., Li, D., Zhu, Y., Wang, Q., & Li, W. (2018). Pathogen-specific Binding soluble Down syndrome cell adhesion Molecule (Dscam) regulates Phagocytosis via Membrane-Bound Dscam in crab. Frontiers in immunology, 9, 801.
- Chen, Y., Li, M., Zhang, Y., Di, M., Chen, W., Liu, X., & Zhang, M. (2018). Traditional Chinese medication Tongxinluo attenuates apoptosis in ox-LDL-stimulated macrophages by enhancing Beclin-1-induced autophagy. Biochemical and biophysical research communications.
- Bashir, K. M. I., Kim, M. S., Stahl, U., & Cho, M. G. (2018). Agrobacterium-mediated genetic transformation of Dictyosphaerium pulchellum for the expression of erythropoietin. Journal of Applied Phycology, 1-16.
- Wang, C., Chen, W. J., Wu, Y. F., You, P., Zheng, S. Y., Liu, C. C., ... & Borjigin, U. (2018). The extent of liver injury determines hepatocyte fate toward senescence or cancer. Cell death & disease, 9(5), 575. Guo, Q., Wu, Y., Hou, Y., Liu, Y., Liu, T., Zhang, H & Teng, W. (2018). Cytokine Secretion and Pyroptosis of Thyroid Follicular Cells Mediated by Enhanced NLRP3, NLRP1, NLRC4, and AIM2 Inflammasomes Are Associated With Autoimmune Thyroiditis. Frontiers in immunology, 9.
- Guo, Q., Wu, Y., Hou, Y., Liu, Y., Liu, T., Zhang, H., & Teng, W. (2018). Cytokine Secretion and Pyroptosis of Thyroid Follicular Cells Mediated by Enhanced NLRP3, NLRP1, NLRC4, and AIM2 Inflammasomes Are Associated With Autoimmune Thyroiditis. Frontiers in immunology, 9.
- Maejima, H., Ninuma, S., Okuda, A., Inoue, T., & Hayashi, M. (2018). Exercise and low-level GABAA receptor inhibition modulate locomotor activity and the expression of BDNF accompanied by changes in epigenetic regulation in the hippocampus. Neuroscience Letters.
- Jiang, Q., Liu, H., Wang, S., Wang, J., Tang, Y., He, Z., & Liang, C. (2018). Circadian locomotor output cycles kaput accelerates atherosclerotic plaque formation by upregulating plasminogen activator inhibitor-1 expression. Acta Biochimica et Biophysica Sinica
- Zhang, J., Cai, H., Sun, L., Zhan, P., Chen, M., Zhang, F., ... & Wan, J. (2018). LGR5, a novel functional glioma stem cell marker, promotes EMT by activating the Wnt/β-catenin pathway and predicts poor survival of glioma patients. Journal of Experimental & Clinical Cancer Research, 37(1), 225.
- Zhang, M., Fu, Z., Chen, J., Zhu, B., Cheng, Y., & Fu, L. (2018). Low level expression of the Mitochondrial Antiviral Signaling protein (MAVS) associated with long-term nonprogression in SIV-infected rhesus macaques. Virology Journal, 15(1), 159.
- Liang, Y., Zhang, T., Shi, M., Zhang, S., Guo, Y., Gao, J., & Yang, X. Low expression of NCOA5 predicts poor prognosis in human cervical cancer and promotes proliferation, migration, and invasion of cervical cancer cell lines by regulating notch3 signaling pathway. Journal of Cellular Biochemistry.
- Zhang, X. M., Huang, Y., Cong, X., Qu, L. H., Zhang, K., Wu, L. L., ... & Yu, G. Y. Parasympathectomy increases resting secretion of the submandibular gland in minipigs in the long term. Journal of Cellular Physiology.
- Ding, M., Zhang, H., Liu, L., & Liang, R. Effect of NOS1 regulating ABCG2 expression on proliferation and apoptosis of cervical cancer cells. Oncology Letters.
- Fu, Y., Dong, J., Wang, J., You, M., Wei, L., Fu, H., ... & Chen, J. (2018). Developmental Exposure to Di-(2-ethylhexyl) Phthalate Induces Cerebellar Granule Cell Apoptosis via the PI3K/AKT Signaling Pathway. Experimental Neurobiology, 27(6), 472-488.
- Wang, Z., Fang, K., Wang, G., Guan, X., Pang, Z., Guo, Y., ... & Wang, F. (2019). Protective effect of amygdalin on epithelial–mesenchymal transformation in experimental chronic obstructive pulmonary disease mice. Phytotherapy Research.
- Coskun, E., Tuna, G., Jaruga, P., Tona, A., Erdem, O., & Dizdaroglu, M. (2019). Identification and quantification of DNA repair protein poly (ADP ribose) polymerase 1 (PARP1) in human tissues and cultured cells by liquid chromatography/isotope-dilution tandem mass spectrometry. DNA Repair.
- Li, K., Meng, Z., Jiang, L., Xia, C., Xu, K., Yuan, D., ... & Liu, S. (2019). CDKL1 promotes the chemoresistance of human oral squamous cell carcinoma cells to hydroxycamptothecin. Molecular and Cellular Probes.
- Zhang, S. H., Liu, D. X., Wang, L., Li, Y. H., Wang, Y. H., Zhang, H., ... & Li, B. (2019). A CASPR1–ATP1B3 protein interaction modulates plasma membrane localization of Na+/K+-ATPase in brain microvascular endothelial cells. Journal of Biological Chemistry, jbc-RA118.
- Wang, Y., Liu, C., & Hu, L. (2019). Cholesterol regulates cell proliferation and apoptosis of colorectal cancer by modulating miR-33a-PIM3 pathway. Biochemical and Biophysical Research Communications.
- Zhang, J., Che, L., Sun, W., Shang, J., Hao, M., & Tian, M. (2019). Correlation of OGR1 with proliferation and apoptosis of breast cancer cells. Oncology Letters, 17(5), 4335-4340.
- Zhang, H. L., Wang, P., Lu, M. Z., Zhang, S. D., & Zheng, L. (2019). c‑Myc maintains the self‑renewal and chemoresistance properties of colon cancer stem cells. Oncology Letters, 17(5), 4487-4493.
- Zhu, Y., Shao, Y., Qu, X., Guo, J., Yang, J., Zhou, Z., & Wang, S. (2019). Sodium ferulate protects against influenza virus infection by activation of the TLR7/9-MyD88-IRF7 signaling pathway and inhibition of the NF-κB signaling pathway. Biochemical and Biophysical Research Communications.
- Misra, S. K., Wu, Z., Ostadhossein, F., Ye, M., Boateng, K., Schulten, K., ... & Pan, D. (2019). Pro-nifuroxazide Self-Assembly Leads to Triggerable Nanomedicine for Anti-Cancer Therapy. ACS applied materials & interfaces.
- Li, X., Inoue, T., Hayashi, M., & Maejima, H. (2019). Exercise enhances the expression of brain-derived neurotrophic factor in the hippocampus accompanied by epigenetic alterations in senescence-accelerated mice prone 8. Neuroscience Letters.
-
Zhang, B., Wang, P. P., Hu, K. L., Li, L. N., Yu, X., Lu, Y., & Chang, H. S. (2019). Antidepressant-Like Effect and Mechanism of Action of Honokiol on the Mouse Lipopolysaccharide (LPS) Depression Model. Molecules, 24(11), 2035.
- Zhu, D., Zhao, J., Lou, A., Huang, Q., OuYang, Q., Zhu, J., ... & Yang, M. (2019). Transforming growth factor β1 promotes fibroblast-like synoviocytes migration and invasion via TGF-β1/Smad signaling in rheumatoid arthritis. Molecular and Cellular Biochemistry, 1-10.
- Li, Q., Zhou, J., Lin, L., Zhao, H., Miao, L., & Pan, Y. (2019). Porphyromonas gingivalis degrades integrin β1 and induces AIF-mediated apoptosis of epithelial cells. Infectious Diseases, 1-9.
- Hua, H., Yang, T., Huang, L., Chen, R., Li, M., Zou, Z., ... & Liu, Y. (2019). Protective effects of lanosterol synthase up-regulation in UV-B induced oxidative stress. Frontiers in Pharmacology, 10, 947.
- Shen, L., Li, C., Zhang, H., Qiu, S., Fu, T., & Xu, Y. (2019). Downregulation of miR-146a Contributes to Cardiac Dysfunction Induced by the Tyrosine Kinase Inhibitor Sunitinib. Frontiers in Pharmacology, 10, 914.
- Liu, S. B., Qiu, X. Q., Guo, W. Q., Li, J. L., Su, Q., Du, J. H., ... & Xu, X. B. (2019). Transcriptome Analysis of FEN1 Knockdown HEK293T Cell Strain Reveals Alteration in Nucleic Acid Metabolism, Virus Infection, C ell Morphogenesis and Cancer Development. Combinatorial chemistry & high throughput screening, 22(6), 379-386.
- Gao, Y., Gui, F., Li, D., Zhang, R., Sun, Q., & Guo, X. (2019). Fluoride regulates the expression of extracellular matrix HSPG and related signaling pathways FGFR3 and Ihh/PTHrP feedback loop during endochondral ossification. Environmental Toxicology and Pharmacology, 103275.
- Xu, W., Sun, D., Wang, Y., Zheng, X., Li, Y., & Xia, Y. (2019). Inhibitory effect of microRNA-608 on lung cancer cell proliferation, migration, and invasion by targeting BRD4 through the JAK2/STAT3 pathway. Bosnian Journal of Basic Medical Sciences.
- Li, J. L., Wang, J. P., Chang, H., Deng, S. M., Du, J. H., Wang, X. X., ... & Song, Y. H. (2019). FEN1 inhibitor increases sensitivity of radiotherapy in cervical cancer cells. Cancer medicine.
- Wang, M., Mingjiang, Y., Liu, J., Norio, T., Bin, Y., Miao, Z., ... & Fangze, T. (2019). Ligusticum chuanxiong exerts neuroprotection by promoting adult neurogenesis and inhibiting inflammation in the hippocampus of ME cerebral ischemia rats. Journal of Ethnopharmacology, 112385.
- Li, B., Li, P., & Bi, L. (2019). Iguratimod dose dependently inhibits the expression of citrullinated proteins and peptidylarginine deiminases 2 and 4 in neutrophils from rheumatoid arthritis patients. Clinical Rheumatology, 1-9.
- Wang, J., Luo, J., Huang, W., Liu, C., Zeng, D., Liu, H., ... & Qin, X. (2019). Increased intracellular Cl− concentration by activating FAK promotes airway epithelial BEAS-2B cells proliferation and wound healing. Archives of Biochemistry and Biophysics, 108225.
- Li, W. J., Gong, Y. L., Gou, W., Li, B., Wang, R., & Lin, Z. F. (2019). Two Cd (II)-based coordination polymers constructed from different donor ligands: structural insights and anti-ovarian cancer activity evolution. Inorganic and Nano-Metal Chemistry, 1-7.
- Yang, L., Wang, B., Qiu, L., Wan, B., Yang, Y., Liu, M., ... & Ye, G. (2020). Functional Characterization of a Venom Protein Calreticulin in the Ectoparasitoid Pachycrepoideus vindemiae. Insects, 11(1), 29.
-
Hu, M., Li, L., Liu, S., Lou, Y., Wang, L., Le, F., ... & Jin, F. Decreased expression of MRE11 and RAD50 in testes from humans with spermatogenic failure. Journal of Assisted Reproduction and Genetics, 1-10.
-
Inoue, T., Okamura, M., Kitahara, M., Takamatsu, Y., Sakakima, H., & Maejima, H. (2020). Exercise plus pharmacological neuromodulation of synaptic inhibition enhance motor function recovery after ischemic stroke. Neuroscience.
- Li, D., Li, L. F., Zhang, Z. F., Zhang, L. P., Liu, Y., & Pan, L. (2020). Synthesis, characterization and anti-lung cancer activity of two transition metal coordination polymers based on the imidazole-containing ligands. Inorganic and Nano-Metal Chemistry, 1-7.
- Itoh, T., Fukatani, K., Nakashima, A., & Suzuki, K. (2020). MicroRNA-141-3p and microRNA-200a-3p regulate α-melanocyte stimulating hormone-stimulated melanogenesis by directly targeting microphthalmia-associated transcription factor. Scientific Reports, 10(1), 1-11.
- Yi, X., Wu, L., Liu, J., Qin, Y. X., Li, B., & Zhou, Q. Low‐intensity pulsed ultrasound protects subchondral bone in rabbit temporomandibular joint osteoarthritis by suppressing TGF‐β1/Smad3 pathway. Journal of Orthopaedic Research®.
- Han, F., Cao, S., Ding, H., Chen, Z., Zhang, Q., & Xu, S. D. (2020). Two mixed-ligand Cd (II)-coordination polymers: structural diversity and antitumor activity against human osteogenic sarcoma cells. Inorganic and Nano-Metal Chemistry, 1-8.
- Han, L., Cao, X., Chen, Z., Guo, X., Yang, L., Zhou, Y., & Bian, H. (2020). Overcoming cisplatin resistance by targeting the MTDH-PTEN interaction in ovarian cancer with sera derived from rats exposed to Guizhi Fuling wan extract. BMC Complementary Medicine and Therapies, 20(1), 1-14.
- Kanasaki, A., Iida, T., Murao, K., Shirouchi, B., & Sato, M. (2020). d-Allulose enhances uptake of HDL-cholesterol into rat’s primary hepatocyte via SR-B1. Cytotechnology, 1-7.
- Ma, L., Chen, X., Zhao, B., Shi, Y., & Han, F. (2020). Enhanced apoptosis and decreased AMPA receptors are involved in deficit in fear memory in Rin1 knockout rats. Journal of Affective Disorders
- Zhou, X. T., Ding, J., Li, H. Y., Zuo, J. L., Ge, S. Y., Jia, H. L., & Wu, J. Hedgehog signalling mediates drug resistance through targeting TAP1 in hepatocellular carcinoma. Journal of Cellular and Molecular Medicine.
- Wang, B., Wang, L., Gu, S., Yu, Y., Huang, H., Mo, K., ... & Liu, C. (2020). D609 protects retinal pigmented epithelium as a potential therapy for age-related macular degeneration. Signal Transduction and Targeted Therapy, 5(1), 1-12.
- Han, H., Song, J. B., Li, C., Zhang, Z. F., An, G. F., & Lin, Z. F. (2020). Synthesis, structures and anticancer activities of two coordination polymers based on 1, 4-bis ((1H-benzo [d] imidazol-1-yl) methyl) benzene ligand. Main Group Chemistry, 19(1), 31-39.
- Song, X., Zhang, B., Taorong, W., Wang, G., Huang, Y., Zhang, Y., ... & Chen, X. (2020). Effects of Cyclopamine on the Viability of Articular Chondrocytes in Rats with Adjuvant Arthritis in vitro. Annals of Clinical & Laboratory Science, 50(1), 85-91.
- Sun, J. L., Zhang, H. Z., Liu, S. Y., Lian, C. F., Chen, Z. L., Shao, T. H., ... & Zhang, W. (2020). Elevated EPSTI1 promote B cell hyperactivation through NF-κB signalling in patients with primary Sjögren’s syndrome. Annals of the Rheumatic Diseases, 79(4), 518-524.
- An, J. R., Zhao, Y. S., Luo, L. F., Guan, P., Tan, M., & Ji, E. S. (2020). Huperzine A, reduces brain iron overload and alleviates cognitive deficit in mice exposed to chronic intermittent hypoxia. Life Sciences, 117573.
- Hou, J., Chen, S. N., Gan, Z., Li, N., Huang, L., Huo, H. J., ... & Nie, P. (2020). In Primitive Zebrafish, MHC Class II Expression Is Regulated by IFN-γ, IRF1, and Two Forms of CIITA. The Journal of Immunology.
- Yang, X., Zhao, C., Sana’a, A. M., Xu, P., Yu, M., Wu, J., ... & Deng, Z. (2020). A chloride channel in rat pancreatic acinar AR42J cells is sensitive to extracellular acidification and dependent on ROS. Biochemical and Biophysical Research Communications.
- Zhang, Y. H., Wang, N., Ma, J., Kang, S., Fan, X. M., & Zhou, X. (2020). Two Cu (II) and Co (II)-based coordination polymers based on the N, S-mixed donor ligand: structural characterization and anti-endometrial cancer activity. Inorganic and Nano-Metal Chemistry, 1-7.
- Ma, Q., Li, J., Zhou, H., Tong, W., & Chen, Y. The function of RNase L and its degradation mechanism in cardiac acute ischemic injury.
- Zhang, J., Zhang, M., Yang, Z., Huang, S., Wu, X., Cao, L., ... & Gao, F. (2020). PDCD4 deficiency ameliorates left ventricular remodeling and insulin resistance in a rat model of type 2 diabetic cardiomyopathy. BMJ Open Diabetes Research and Care, 8(1), e001081.
-
Yang, X., Zhao, C., Sana’a, A. M., Xu, P., Yu, M., Wu, J., ... & Deng, Z. (2020). A chloride channel in rat pancreatic acinar AR42J cells is sensitive to extracellular acidification and dependent on ROS. Biochemical and Biophysical Research Communications.
Description
Minute TM Total Protein Extraction Kit for Animal Cultured Cells/Tissues
Minute™ Total Protein Extraction Kit for Animal Cultured Cells and Tissues is the most advanced tool for rapid protein extraction without altered protein profile. More and more evidence has shown that the most commonly used RIPA buffer can cause unpredictable protein loss, resulting in questionable data interpretation. This problem is fully resolved by the patented spin-column-based technologies, which, when used with stronger lysis buffers, can effectively extract proteins from tough tissues such as skin, blood vessels, muscle, etc. The use of the proprietary protein extraction filter cartridges can be found extremely helpful When starting material is a limiting factor, allowing the extraction volume to be as low as 20 µl. The kit provides both denaturing and native cell lysis buffers, so users can select according to specific applications. Total proteins can be extracted from cultured cells/tissues in 1-8 minutes with high yield (2-8 mg/ml).
Selected references related to protein loss by RIPA buffer:
- Bai, B., and Laiho, M. (2012) Proteomics. 12:3044-3048
- Mukhopadhyay, C. et al. (2016) PNAS 5:8228-8237
- Li, Q. (2016) Biotechniques. 61:327
- Ngoka, L. CM. (2008) Proteome Science. 6:30
Protein profiles of animal tissues extracted by denaturing lysis buffer and native lysis buffer. Extracted proteins were separated in 10% SDS-PAGE and stained with coomassie blue. Lane 1, drosophila larvae; lane 2, drosophila pupa; 3, adult drosophila; 4, goldfish muscle and 5, mouse liver. 
Kit includes:
|
Items |
Quantity |
|
Denaturing Cell Lysis Buffer |
25 ml |
|
Native Cell Lysis Buffer |
25 ml |
|
Protein Extraction Filter Cartridges |
50 units |
|
Collection Tubes with Caps |
50 units |
|
Plastic Rods |
2 units |
Delivery Information
Payment & Security
Your payment information is processed securely. We do not store credit card details nor have access to your credit card information.