Antibodies

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Western blot analysis on HeLa cell lysate using p14 ARF Antibody. The lane on the left is treated with the antigen-specific peptide.AF0229 at 1/200 staining human Breast cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondary
Western blot analysis on HeLa cell lysate using p16 INK Antibody. The lane on the left is treated with the antigen-specific peptide.AF0228 at 1/200 staining human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondary
Western blot analysis on mouse brain lysate using Osteopontin AntibodyAF0227 staining LOVO cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Cat.# S0006), diluted at 1/600, was used as secondary antibod
Western blot analysis on HeLa cell lysate using OCT3 Antibody. The lane on the left is treated with the antigen-specific peptide.This image is a courtesy of anonymous review.
AF0225 staining Daudi cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Cat.# S0006), diluted at 1/600, was used as secondary antibodAF0225 staining COS7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod
AF0224 staining Hela by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. Samples were then incubated with primary Ab(AF0224 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37¡ãC. An  AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(S0006 1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(S0017 1:600 Green) were used as the secondary antibodWestern blot analysis of OCT1  expression in Mouse brain lysate
Western blot analysis on HepG2 cell lysate using NSE Antibody. The lane on the left is treated with the antigen-specific peptide.AF0223 at 1/100 staining human brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondary
Western blot analysis on K562 cell lysate using NM23 Antibody. The lane on the left is treated with the antigen-specific peptide.AF0222 staining K562 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod
Western blot analysis of extracts from human prostate, using NKX3.1 Antibody.AF0221 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondary
Western blot analysis on HepG2 cell lysate using MMP9 Antibody. The lane on the left is treated with the antigen-specific peptide.IHC analysis of Mouse liver tissue, using MMP9 Antibody at 1/100
Western blot analysis on NIH-3T3 cell lysate using MMP8 Antibody. The lane on the left is treated with the antigen-specific peptide.AF0219 at 1/100 staining human bone tissue sections by IHC-P. The tissue was   formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was   performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22  ¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondary
Western blot analysis of MMP7 expression in Mouse brain lysateIHC analysis of mouse colon tissue, using MMP7 Antibody at 1/100.
Western blot analysis on 293 cell lysate using MMP3 Antibody. The lane on the left is treated with the antigen-specific peptide.AF0217 staining Hela by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. Samples were then incubated with primary Ab(AF0217 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37¡ãC. An  AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(S0006 1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(S0017 1:600 Green) were used as the secondary antibod
AF0216 staining SKOV3 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Cat.# S0006), diluted at 1/600, was used as secondary antibodWestern blot analysis of extracts from various samples, using MMP23 Antibody.
 Lane 1: Hela treated with blocking peptide.
 Lane 2: Hela;
 Lane 3: Hepg2;
Western blot analysis on HuvEc cell lysate using MMP19 Antibody. The lane on the left is treated with the antigen-specific peptide.AF0215 at 1/100 staining human liver carcinoma tissue sections by IHC-P. The tissue was   formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was   performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22  ¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondary
Western blot analysis on HepG2 cell lysate using MMP16 Antibody. The lane on the left is treated with the antigen-specific peptide.AF0214 at 1/100 staining human testis tissue sections by IHC-P. The tissue was   formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was   performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22  ¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondary
Western blot analysis on MDA-MB-435 cell lysate using MMP15 Antibody. The lane on the left is treated with the antigen-specific peptide.AF0213 staining MDA-MB-435 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod
AF0212 at 1/200 staining human Oral squamous cell carcinoma sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondaryAF0212 at 1/200 staining human Oral squamous cell carcinoma sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondary
AF0211 at 1/100 staining human liver carcinoma tissue sections by IHC-P. The tissue was   formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was   performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22  ¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondaryAF0211 staining A549 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod
Western blot analysis of extracts from Hela, using MMP1 Antibody. Lane 1 was treated with the antigen-specific peptide.AF0209 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod
Western blot analysis on COS7 cell lysate using MDM2 Antibody. The lane on the left is treated with the antigen-specific peptide.This image is a courtesy of anonymous review
AF0207 at 1/100 staining human bone tissue sections by IHC-P. The tissue was   formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was   performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22  ¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondaryAF0207 staining HeLa  cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Cat.# S0006), diluted at 1/600, was used as secondary antibod
AF0206 at 1/100 staining human colon carcinoma tissue sections by IHC-P. The tissue was   formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was   performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22  ¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondaryAF0206 staining HeLa  cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Cat.# S0006), diluted at 1/600, was used as secondary antibod
Western blot analysis on COS7 cell lysate using MCL1 Antibody. The lane on the left is treated with the antigen-specific peptide.IHC analysis of mouse colon tissue, using MCL1 Antibody at 1:100.

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