PiNK Plus Prestained Protein Ladder 500ul

PiNK Plus Prestained Protein Ladder

500ul

• Broad range: 10 - 175 kDa.
• Ready-to-use: supplied in a loading buffer for direct loading on gels.
• Easy to identify: includes the ~10, ~40 and ~90 kDa reference bands coupled with a blue dye.
• Sharp bands.

The PiNK Plus Prestained Protein Ladder contains 11 proteins that resolve into sharp, tight bands in the range of 10-175 kDa. The PiNK Plus Prestained Protein Ladder allows you to monitor molecular weight separation during electrophoresis, estimate molecular weights of proteins of interest, and evaluate western transfer efficiency.

Contents : 
Approximately 0.2~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 1 mM Dithiothreitol, 4.8 M Urea, and 12 % (v/v) Glycerol.

Quality Control : 
Tested by SDS-polyacrylamide gel electrophoresis and Western blotting.

Storage : 
Stable for up to 2 weeks at 25°C.
Stable for up to 3 months at 4°C.
Stable for up to 24 months at -20°C.

Recommendations for Loading : 
•  Thaw the ladder either at room temperature or at 37-40°C for a few minutes to dissolve precipitated solids. Do not boil.
•  Mix thoroughly to ensure the solution is homogeneous.
•  Load the following volumes of the ladder on the SDS-polyacrylamide gel :
  5 μl per well for mini-gels, 2.5 μl per well for blots
10 μl per well for large gels, 5 μl per well for blots

Protocol

SDS